The Bacteriological And Mycological Contamination Of Domestic Water
[A CASE STUDY OF MALETE COMMUNITY, ILORIN, KWARA STATE.]

b.    Confirmative Test
• A loopful of sample from positive tubes was transferred to an Eosin Methylene Blue (EMB)agar tube under aseptic condition and the tubes marked as in the earlier test.
• The tubes were incubated370C for 48 hours
• After incubation, positive tubes were recorded indicated by the presence of blue black with metallic sheen colonies in the media.
• The reading was converted to MPN index / 100 ml using MPN index table presented in the Standard Methods.
Detection of Salmonella species was done by the enrichment of water samples on Selenite F broth, followed by isolation of the typical organism on selective medium, Xylose Lysine Deoxycholate Agar (XLD) and Kieglar Iron Agar (KIA). Detection of Vibrio cholerae was done by enriching the samples in 1% alkaline peptone water for 6 to 8 hours followed by isolation on Thiosulphate Citrate Bile salt sucrose (TCBS) agar medium (Collee et al., 2009). For Pseudomonas aeruginosa both MacConkey agar, Nutrient agar were used as presumptive cultures and Blood agar were used to isolate Staphylococcus aureus (Benson, 2008). All colonies with different characteristics on their selective media were identified on the basis of their colonial, morphological and biochemical properties following Bergey’s Manual of Determinative Bacteriology (Holt et al., 2014).
The fungi were isolated from the water samples seasonally by using two methods: The direct plate and the dilution plate, two types of growth media were used for isolation of fungi, Potato Dextrose Agar (PDA) and Sabouraud Dextrose Agar (SDA) supplemented with chloramphenicol (50 mg/l) and cycloheximide (500 mg/l) and examined for fungal growth over a period of four weeks.
3.3.2    Identification of Bacteria and Fungi Isolates
The identification of the bacterial isolates was accomplished by the observation of colonial characteristics, Gram reaction and biochemical tests such as Oxidase test, Catalase test, Citrate test, Urease test, Coagulase test, TSI (Triple Sugar Iron agar) test, MIO (Motility Indole Ornithine) test and Methyl Red and Vogesproskauer test. Identification of fungi isolate was carried out by adding a drop of lacto-phenol on cotton blue stain placed on a clean grease slide and a sterile inoculating needle used to tease out a fragment of the fungi and transferred into the stain on the slide and covered with a cover slip and examined with low power objective of the microscope x 40.
3.4    Statistical analysis
Statistical analysis was conducted for the data using Statistical Package for the Social Sciences software program, SPSS version 20 (SPSS Inc., USA) to calculate the mean and Standard Deviation [SD]. The Student’s T-test and Analysis of Variance [ANOVA] test, Post hoc test (Duncan) was applied to determine significant differences in spatial and in temporal variation. All data are expressed as mean ± SE. A p-value of 0.05 was considered as the limit for statistical significance.