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The Effect Of Antidiabetic Agent Glibenclamide And Meltformine On Lipids And Glycated Haemoglobin In Type 2 Diabetes Patient Attending Uith Ilorin
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Step 2
100µl of the supernatant was dispensed into the clean test tubes respectively.
2ml of the cholesterol reagent was added
It was incubated at room temperature for 10mins
Absorbance of sample against reagent blank was measured at 505nm
Glycated Haemoglobin
Glycated Haemoglobin is a form of haemoglobin that is measured primarily to identify the three-month average plasma glucose concentration. The test is limited to a three-month average.
Procedure
ReagentsBlank(µl) sample/standard(µl)
R3 500
Whole blood 10
Incubated for 5mins
Add 180µl of R1 into HbA1C tubes
Add 5µl of hemolysed sample into HbA1C tubes
It was pressed, entered twice of the haemoglobin HbA1C until it makes sound
R2: 60µl into it
Read and result was documented.
Fasting Blood Sugar
Principle: Glucose oxidase catalyse the oxidase of glucose to produce hydrogen peroxide and gluconic acid. Hydrogen peroxide in the presence of enzymes, peroxidase breakdown and oxygen give off the reaction with 4-amino phenozone and phenol to give a pink colour.
Method: Determination of plasma glucose by Glucose Oxidase Peroxidase method.
Procedure
ReagentsBlank(µl) sample/standard(µl)
Sample 20
Reagent (R1 and R2) 2mls
Incubate at room temperature for 20mins
Measure the absorbance of standard and sample against reagent blank at 520nm
Triglycerides
Principle: Triglycerides in the sample are hydrolyzed to glycerol and free fatty acids by the action of lipase, the change in absorbance is directly proportional to the concentration of triglyceride in the sample. Triglyceride measurement is used in the diagnosis and treatment of lipid and other disorders.
Procedure
1 20µl of sample/control/standard were pipetted into clean test tube and labelled appropriately.
2 2ml of working reagent was added.
3 It was mixed and incubated for 10mins at room temperature.
4 The absorbance of sample and standard was measured against reagent blank at 505nm.
The calculation was done
Triglyceride concentration (mmol/l) = Absorbance of sample
Absorbance of standard
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ABSRACT - [ Total Page(s): 1 ]Abstract Is Coming Soon ... Continue reading---
APPENDIX A - [ Total Page(s): 1 ]APPENDIX IQUESTIONAIRE TO ACCESS THE ANTHROPOLOGIC INDICES OF PATIENTS WITH TYPE TWO DIABETES MELLITUS ON ANTIDIABETIC DRUGS (METFORMIN AND GLIBENCLAMIDE) ATTENDING UITH ILORIN.INTRODUCTION: I am a final year students of the Department of Medical Laboratory Science, School of Basic Medical Sciences, Kwara State University, Malete, Kwara State. This questionnaire is aimed at accessing the demographic indices of patients with type 2 Diabetes mellitus on metformin and diabinese in Ilorin metropolis ... Continue reading---
CHAPTER ONE - [ Total Page(s): 2 ]The present study was designed to investigate and compare the effects of glibenclamide and metformin on prevalence of metabolic syndrome in type 2 diabetic patients.1.2 STATEMENT OF PROBLEMTo know if antidiabetic agents glibenclamide and meltformine has any effect on lipid and glycated haemoglobin in type 2 diabetes patients1.3 AIM OF STUDYTo evaluate the effect of antidiabetic agent glibenclamide and meltformine on lipids and glycated haemoglobin in type 2 diabetes patient attendi ... Continue reading---
CHAPTER TWO - [ Total Page(s): 11 ]Triglycerides as major components of very low-density lipoprotein (VLDL) and chylomicrons, play an important role in metabolism as energy sources and transporters of dietary fat. They contain more than twice as much energy (approximately 9kcal/g or 38kj/g) as carbohydrates (approximately 4kcal/g or 17kj/g) (Brefere et al., 2014).2.5 Glycated hemoglobin (hemoglobin A1c, HbA1c, A1C, or Hb1c; sometimes also referred to as being Hb1c or HGBA1C) is a form of hemoglobin that is measured primarily to i ... Continue reading---
CHAPTER THREE - [ Total Page(s): 1 ]CHAPTER THREE3.1 Material and Method3.2 Study AreaThe study was carried out at University of Ilorin Teaching Hospital, Ilorin, Kwara State. The hospital is located at the State capital of Ilorin, Kwara State Nigeria. It is a referral center to other public and private hospitals within and outside the state.3.3 SAMPLE SIZE DETERMINATIONThere was a random selection of ninety (90) subjects, 60 were type 2 Diabetes mellitus individual using either one or combine antidiabetic agent (glibe ... Continue reading---
CHAPTER FOUR - [ Total Page(s): 4 ]Tables 4.6: Correlation of Duration in Diabetes and BMI with biochemical parameters (T. cholesterol, High Density Lipoprotein, Low Density Lipoprotein, triglycerides, glycated, and fasting blood sugar) in Diabetic patient using antidiabetic drugs (Metformin and Glianpride). ... Continue reading---
CHAPTER FIVE - [ Total Page(s): 2 ]CHAPTER FIVE5.0 DISCUSSIONThe study shows discrepant results about the influence of metformin on lipid profile (10). Some studies, in agreement with ours, reported reduction only in TC levels (Grant, 1996; Ginsberg et al., 1999), while others reported reduction of TC and TG with an increase of HDL-C (Robinson et al., 1998; Yki-Jarvinen et al., 1999). Still other studies showed no changes in lipid profile (Groop et al., 1998; Rains et al., 1998). Another investigation showed an association of met ... Continue reading---
REFRENCES - [ Total Page(s): 3 ]Rodger, W. (2012). Sulphonylureas and heart disease in diabetes management. Diabetes Spectrum. Pg. 12–27.Rosenbaum, M. and Leibel, R. L. (2014). Role of leptin in energy homeostasis in humans. Journal of Endocrinology. 223(1): 83-96.Rowley, D.E. and Bezold, D.C. (2012). Using new insulin strategies in the outpatient treatment of diabetes: clinical applications. Journal of American Medical Association. Pg. 289.Shaw, D., De Rosa, N. and Di Maro, G. (2010). Metformin improves glucose, lipid ... Continue reading---
