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ISOLATION AND IDENTIFICATION OF CRUDE OIL DEGRADING BACTERIA FROM SOIL POLLUTED WITH AUTOMOBILE LUBRICANTS
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ABSRACT - [ Total Page(s): 1 ]Oil spillage is a big problem in most industries especially in developing countries and it contaminates soil and water. Microorganisms can be used to remediate crude oil contaminated sites through bioremediation. Bioremediation is preferred to other methods of decontamination of polluted soil because these other methods lead to production of toxic compounds and are non-economic.
The aim of this study was to isolate and identify crude oil degrading bacteria from soil polluted with automobile lubricants, determine their rate of crude oil utilization and select the best degraders. Oil contamin
... Continue Reading
TABLE OF CONTENTS - [ Total Page(s): 1 ]Title page Approval page Dedication Acknowledgement Abstract Table of content List of tables List of figures CHAPTER ONE – INTRODUCTION AND LITERATURE REVIEW Introduction Literature review Component of crude oil Crude oil degrading bacteria Bacteria Fungi and Yeasts Algae Biodegradation and Bioremediation Physicochemical versus biological methods for degrading petroleum hydrocarbon pollutants Microbial degradation of pe ... Continue Reading
CHAPTER ONE - [ Total Page(s): 2 ]INTRODUCTION AND LITERATURE REVIEW INTRODUCTION Crude oil is composed of several compounds, such as aliphatic, aromatic and polyaromatic hydrocarbon, and also Sulphur, oxygen and nitrogen containing components. These compounds are toxic and can cause cancer. Oil waste management in oil refineries needs to be focused on administering waste and selecting appropriate strategies in order to prevent soil damage. Hydrocarbon components have been known to belong to the family of carcinogens and neurotoxic organic pollutants (Nilanjana and Preethy, 2011). Petroleum products ... Continue Reading
CHAPTER TWO - [ Total Page(s): 1 ] MATERIALS AND METHODS 2.0. MATERIALS 2.2. SAMPLE COLLECTION The soil contaminated with used motor oil collected from Mechanic Village, Nsukka, Enugu state. The lubricating oil was collected from total filling station, Nsukka, Enugu state. The kerosene was collected from Mobil filling state, Nsukka, Enugu state. 2.2. REAGENTS AND MEDIA Nutrient agar, agar-agar, inorganic salts, casein, peptone, phenol red, sucrose, maltose. Lactose, dextrose, beef extract, mannitol, bromothymol blue, glucos ... Continue Reading
CHAPTER THREE - [ Total Page(s): 1 ]RESULTS Six distinct colonies were isolated from the Bushnell Haas agar and subjected to several biochemical tests. Two isolates were found to be the best crude oil degraders by measuring their optical densities at 600nm wavelength using spectrophotometer. The two best isolates were identified, and they were subjected to belong to the genera Micrococcus and Streptomyces. Table 1 shows the morphological characteristics of the isolates. Table 2 shows the microscopy results of the isolates. Table 3 shows the morphological and biochemical test results of the selected isolates. Tab ... Continue Reading
CHAPTER FOUR - [ Total Page(s): 1 ]DISCUSSION AND CONCLUTION 4.1. DISCUSSION Six distinct colonies were isola ted from Bushnell Haas agar. The cover of the Bushnell Haas agar plates containing filter paper dipped in kerosene; this is to allow only the growth of microorganisms that can utilize kerosene (petroleum product) as sourse of energy and carbon. The six isolates were subjected to several biochemical tests and morphological characterization. Spectrophotometric analysis was carried out to check the optical densities of the different isolates in order to determine rate of crude oil utilization by the isolat ... Continue Reading
REFRENCES - [ Total Page(s): 1 ] REFERENCES Abbasian, F., Lockington, R., Naidu, R. and Mallavarapu, M. (2015). Acomprehensive review of aliphatic hydrocarbon biodegradation by bacteria. Applied Biochemical Biotechnology 1-30. Adenipekin, C. O. (2008), Bioremediation of engine oil polluted soil by pleurotus tuber-regium singer, a Nigeria whole rot funus. African Journal of Biotechnology 7 (1): 055-058. Aislabie, J., Saul, D. and Foght, J. (2006). Bioremediation of hydrocarbon-contaminated polar ... Continue Reading
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ABSRACT - [ Total Page(s): 1 ]Oil spillage is a big problem in most industries especially in developing countries and it contaminates soil and water. Microorganisms can be used to remediate crude oil contaminated sites through bioremediation. Bioremediation is preferred to other methods of decontamination of polluted soil because these other methods lead to production of toxic compounds and are non-economic.
The aim of this study was to isolate and identify crude oil degrading bacteria from soil polluted with automobile lubricants, determine their rate of crude oil utilization and select the best degraders. Oil contamin
... Continue Reading
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