-
The Effects Of Metformin And Diabinese On Female Sex Hormone Of Type 2 Diabetes Mellitus Patients
[UNIVERSITY OF ILORIN TEACHING HOSPITAL (UITH), ILORIN, KWARA STATE] -
-
-
3.8 CALCULATION OF RESULTS
1. The mean absorbance value (A450) for each set of reference standards and samples was calculated.
2. A standard curve was constructed by plotting the mean absorbance obtained for each reference standard against its concentration in pg/ml on a linear-linear graph paper, with absorbance values on the vertical or Y axis, and concentrations on the horizontal or X axis.
3. The mean absorbance values for each specimen was used to determine the corresponding concentration of Estrogen in pg/ml from the standard curve.
3.8.1 DETERMINATION OF PLASMA FASTING GLUCOSE
METHOD: Glucose Oxidase Peroxidase (GOD-POD) according to Trinder, (1969).
PRINCIPLE:
Enzymatic colorimetric determination of glucose according to the following reaction.
Glucose is oxidized by Glucose oxidase and produces gluconate and hydrogen peroxide. The hydrogen peroxide is oxidatively coupled with 4-aminoantipyrine and phenol. The intensity of the coloured complex (quinoneimine) is proportional to the glucose concentration in the sample and can be measured photometrically at 505nm.
Procedure for Glucose estimation:
i. Clean test tubes were labelled as blank standard and sample
ii. 20 µL of test and standard was dispensed into the labelled test tubes respectively.
iii. 2000 µL (2ml) of glucose working reagent was added to all the tubes.
iv. It was mixed and incubated for 20minutes at room temperature
v. Absorbance was read spectrophotometrically at 505nm against reagent blank.
Protocol for estimation of plasma glucose:
Pipette into the cuvettes:
-
-
-
ABSRACT - [ Total Page(s): 1 ]ABSTRACT is coming soon ... Continue reading---
APPENDIX A - [ Total Page(s): 1 ]APPENDIX IQUESTIONNAIRE TO ACCESS THE ANTHROPOLOGIC AND DEMOGRAPHIC INDICES OF PATIENTS WITH TYPE TWO DIABETES MELLITUS ON ANTIDIABETIC DRUGS (METFORMIN AND DIABINESE) ATTENDING UITH ILORIN.INTRODUCTION: I am a final year students of the Department of Medical Laboratory Science, School of Basic Medical Sciences, Kwara State University, Malete, Kwara State. This questionnaire is aimed at accessing the demographic indices of patients with type 2 diabetes mellitus on metformin and diabinese in Ilor ... Continue reading---
APPENDIX B - [ Total Page(s): 1 ]APPENDIX 11REAGENT COMPOSITION FOR ESTROGENEstrogen calibrates, Horseradish Peroxidase Conjugates, Estrogen biotinylated purified rabbit igG conjugates, Tetremethylbenzidine(TMB), wash buffer concentrate, assay buffer, Stop solution.REAGENT COMPOSITION FOR PROGESTERONEProgesterone calibrates, Horseradish Peroxidase Conjugates, Progetarone biotinylated purified rabbit igG conjugates, Tetremethylbenzidine (TMB), wash buffer concentrate, assay buffer, Stop solution. ... Continue reading---
CHAPTER ONE - [ Total Page(s): 2 ]CHAPTER ONEINTRODUCTION1.1 BACKGROUND OF STUDY Diabetes mellitus is a group of metabolic disorders in which a person has high plasma glucose, either because the body does not produce enough insulin, or because cells do not respond to the insulin that is produced. The high plasma glucose produces the classical symptoms of polyuria, polydipsia and polyphagia (Rother, 2007). Type 2 diabetes mellitus, formerly non-insulin dependent diabetes mellitus or adult onset diabetes, is a ... Continue reading---
CHAPTER THREE - [ Total Page(s): 6 ]3.7.2 REAGENT Materials provided with the kit: 1. Antibody-Coated Wells (1 plate, 96 wells) Microtiter wells coated with 1.0 µg/ml streptavidin was packaged in an aluminium.2. Reference Standard Set (1ml/vial) Contains 0, 20, 100, 250, 500 1500,3000 in pg/ml with preservatives.3. Rabbit Anti-estrogen Reagent (6 ml) Contains rabbit anti-estradiol biotinylated rabbit igG conjugate in buffer, yellow dye with preservatives.4. Estradiol enzyme reagent (6 m ... Continue reading---
CHAPTER FOUR - [ Total Page(s): 3 ]Table 4.4: Comparison of biochemical parameters (estrogen, progesterone and fasting blood sugar) between diabetic patients on metformin only and diabetic patients on combined metformin with other drugs (glimepride).Table 4.5 depicts the Pearson correlation of duration of diabetes and BMI with biochemical parameters (estrogen, progesterone and fasting blood sugar) in diabetic patients using antidiabetic drugs (metformin and diabinese).Data indicated a significant correlation between biochemical p ... Continue reading---
CHAPTER FIVE - [ Total Page(s): 2 ]CHAPTER FIVE5.0 DISCUSSIONType 2 diabetes mellitus, formerly known as non-insulin dependent diabetes mellitus or adult onset diabetes, is a metabolic disorder that is characterized by hyperglycemia in the context of insulin resistance and relative insulin deficiency (Vinay et al., 2008). Sex differences and the role of gonadal hormones in modulating insulin sensitivity and glucose tolerance are of increasing interest and importance because of the increasing prevalence of type 2 diabetes me ... Continue reading---
REFRENCES - [ Total Page(s): 4 ]Murali, R. and Saravanan, R. (2012). Antidiabetic effect of d-limonene, a monoterpene in streptozotocin-induced diabetic rats. Biomedical Prevention and Nutrition.2:269-275.Neerati, P., Devde, R., and Gangi, A.K. (2014). Evaluation of the effect of curcumin capsules on glyburide therapy in patients with type-2 diabetes mellitus. Phytotherapeutic. Research. 28:1796-1800.Nelson, L.R. and Bulun, S.E. (2011).Estrogen production and action. Journal of America Academic and Dermatology. 4 ... Continue reading---
-
ABSRACT - [ Total Page(s): 1 ]ABSTRACT is coming soon ... Continue reading---