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Isolation And Identification Of Crude Oil Degrading Bacteria From Soil Polluted With Automobile Lubricants
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DISCUSSION AND CONCLUTION
4.1. DISCUSSION
Six distinct colonies were isola ted from Bushnell Haas agar. The cover of the Bushnell Haas agar plates containing filter paper dipped in kerosene; this is to allow only the growth of microorganisms that can utilize kerosene (petroleum product) as sourse of energy and carbon.
The six isolates were subjected to several biochemical tests and morphological characterization. Spectrophotometric analysis was carried out to check the optical densities of the different isolates in order to determine rate of crude oil utilization by the isolates and select the best degraders. The isolates were inoculated into 100ml of mineral salt broth containing 2ml of lubricating oil and readings were taken immediately after the inoculation, at every 24 hours interval for 7 days.
As the time increases, the medium becomes more turbid and the optical densities increased. Indicated that the microorganisms are utilizing the lubricating oil as source of energy and carbon. The further confirms that the microorganisms are crude oil degraders and it also determines the best degraders. For most of the isolates, the 96th hour readings showed a sharp increase in thptical density indicating the utilization of crude oil at its peak. At the 7th day, the optical density dropped for all the isolates, this indicates that the isolates have used up the crude oil.
The best degraders were selected and identified using the systematic scheme of Bergey’s Manual of syste,atic Bacteriology by comparing the biochemical tests of results and morphological characteristics of the isolates to the various microorganisms’ genera in the manual. The genera, Micrococcus and Streptococcus were observed to be the best degraders.
4.2. CONCLUSION
Different microorganisms can be isolated from soil contaminated with crue oil, but they may not all be crude oil degraders. Also, different microorganisms have different crude oil degradability capacity; some are good degraders while some are average degraders. The study shows that Micrococcus and Streptomyces are good degraders of crude oil and therefore can be used for bioremediation of soil contaminated with crude oil.
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ABSRACT - [ Total Page(s): 1 ]Oil spillage is a big problem in most industries especially in developing countries and it contaminates soil and water. Microorganisms can be used to remediate crude oil contaminated sites through bioremediation. Bioremediation is preferred to other methods of decontamination of polluted soil because these other methods lead to production of toxic compounds and are non-economic.
The aim of this study was to isolate and identify crude oil degrading bacteria from soil polluted with automobile l ... Continue reading---
TABLE OF CONTENTS - [ Total Page(s): 1 ]Title page Approval page Dedication Acknowledgement Abstract Table of content List of tables List of figures CHAPTER ONE – INTRODUCTION AND LITERATURE REVIEW Introduction Literature review Component of crude oil Crude oil degrading bacteria Bacteria Fungi and Yeasts Algae Biodegradation and Bioremediation Physicochemical versus b ... Continue reading---
CHAPTER ONE - [ Total Page(s): 2 ]INTRODUCTION AND LITERATURE REVIEW INTRODUCTION Crude oil is composed of several compounds, such as aliphatic, aromatic and polyaromatic hydrocarbon, and also Sulphur, oxygen and nitrogen containing components. These compounds are toxic and can cause cancer. Oil waste management in oil refineries needs to be focused on administering waste and selecting appropriate strategies in order to prevent soil damage. Hydrocarbon components have been known to belong to the family of ... Continue reading---
CHAPTER TWO - [ Total Page(s): 1 ] MATERIALS AND METHODS 2.0. MATERIALS 2.2. SAMPLE COLLECTION The soil contaminated with used motor oil collected from Mechanic Village, Nsukka, Enugu state. The lubricating oil was collected from total filling station, Nsukka, Enugu state. The kerosene was collected from Mobil filling state, Nsukka, Enugu state. 2.2. REAGENTS AND MEDIA Nutrient agar, agar-agar, inorganic salts, casein, pepton ... Continue reading---
CHAPTER THREE - [ Total Page(s): 1 ]RESULTS Six distinct colonies were isolated from the Bushnell Haas agar and subjected to several biochemical tests. Two isolates were found to be the best crude oil degraders by measuring their optical densities at 600nm wavelength using spectrophotometer. The two best isolates were identified, and they were subjected to belong to the genera Micrococcus and Streptomyces. Table 1 shows the morphological characteristics of the isolates. Table 2 shows the microscopy results of the isolat ... Continue reading---
REFRENCES - [ Total Page(s): 1 ] REFERENCES Abbasian, F., Lockington, R., Naidu, R. and Mallavarapu, M. (2015). Acomprehensive review of aliphatic hydrocarbon biodegradation by bacteria. Applied Biochemical Biotechnology 1-30. Adenipekin, C. O. (2008), Bioremediation of engine oil polluted soil by pleurotus tuber-regium singer, a Nigeria whole rot funus. African Journal of Biotechnology 7 (1): 055-0 ... Continue reading---
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ABSRACT - [ Total Page(s): 1 ]Oil spillage is a big problem in most industries especially in developing countries and it contaminates soil and water. Microorganisms can be used to remediate crude oil contaminated sites through bioremediation. Bioremediation is preferred to other methods of decontamination of polluted soil because these other methods lead to production of toxic compounds and are non-economic.
The aim of this study was to isolate and identify crude oil degrading bacteria from soil polluted with automobile l ... Continue reading---
CHAPTER FOUR -- [Total Page(s) 1]
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CHAPTER FOUR -- [Total Page(s) 1]
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